Lectures given during the FEBS Advanced Courses "Ligand Binding: Theory and Practice" (Nove Hrady, CZ) Hemoglobin and Allostery (FEBS Advanced Course 2014); slides On the Equilibrium Constant of Irreversible Reactions; slides Allostery and Cooperativity (FEBS Advanced Course 2016); slides UV/Vis Absorbance spectroscopy for the study of ligand binding (FEBS Advanced Course 2016); slides Tutorial of the absorbance experiment presented at the FEBS Advanced Course 2016. Some pictures taken during the 2016 FEBS Course A series of interactive tutorial on ligand binding. You can take these tutorials in whatever order but pay attention to the fact that some concept are defined in tutorial #1 and used without definition in the successive tutorials. 1) Ligand binding to a single-site protein 2) Ligand binding incompatible with a single-site protein: case 1 3) Ligand binding incompatible with a single-site protein: case 2 (homotropic linkage) 4) High affinity ligands 5) Very high affinity ligands (in preparation; feel free to explore) 6) Low affinity ligands 7) Ligand replacement reactions (identical linkage) 8) Heterotropic linkage